Vol 49 No. 12: 1122-1128 [PDF] [Full Text]
Time-dependent glucocorticoid administration differently affects peripheral circadian rhythm in rats
Tao Wu and Zhengwei Fu*
College of Biotechnology and Bioengineering, Zhejiang University of Technology, Hangzhou 310014, China

Abstract  There is a growing recognition that glucocorticoid (GC) acts as an internal timing signal for peripheral circadian oscillators. However, the transcription process of GC-related clock gene in the peripheral tissues is not fully understood. The present study was designed to explore the potential role of clock genes in the GC-induced peripheral circadian gene expression in vivo. Real-time RT-PCR analysis indicated that the transcript levels of Per1 and Dec1 were rapidly up-regulated within 0.5 and 1 h in the heart and kidney respectively after stimulation with dexamethasone (Dex). These results suggest that Per1 and Dec1 serve as the primary and secondary responsers respectively in initiating the GC-induced peripheral circadian gene expression. By comparing the effects of the different GC administration schedules on the circadian rhythm of clock genes in peripheral tissues in rats, we found that the circadian phases of Bmal1 and Per1 were shifted more in the ZT0 (endogenous valley time) Dex stimulation group than in the ZT12 (endogenous peak time) Dex stimulation group in heart and kidney under the normal LD cycle. Under the jet lag condition, the circadian phases of Bmal1 and Per1 were also shifted more in the ZT0 Dex stimulation group than in the ZT12 Dex stimulation group. Therefore, the GC stimulation in the endogenous valley time caused circadian disorder in the normal LD cycle, but it might benefit the circadian resetting of peripheral clocks under the LD reversal condition.


Keywords   glucocorticoid, circadian clock, heart, kidney, rat


Received   2017-4-9  


Funding  This work was supported by the grant from the National Natural Science Foundation of China (No. 31200890), the Open Research Fund Program of Collaborative Innovation Center of Membrane Separation and Water Treatment, Zhejiang Province Public Welfare Research Project (No. 2015C33178), and the Scientific Innovation Program for University Students in Zhejiang Province (No. 2014R403060).


* Correspondence address  Tel/Fax: +86-571-88320599; E-mail: azwfu@zjut.edu.cn

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